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Tuesday, 20 January 2026

The dark side of life: new method developed to identify soil organisms

Hands of a person inspecting soil health

The quality of the soil depends on the organisms that inhabit it. Biologists at the University of Graz have developed a new method for identifying tiny creatures. Photo: Kannapat/Adobe Stock

A large number of soil animals are smaller than one millimetre and are therefore difficult to study scientifically. Biologists from the University of Graz have now developed a method to determine both the shape and the DNA of the organisms. The results have been published in the journal Geoderma.

What creeps under our feet plays a key role in agriculture, the ecosystem and the climate. The soil harbours more than half of the world's biodiversity, yet many of the little animals - the so-called dark taxa - have hardly been researched. These include mites and pauropods, for example, which are often much smaller than a millimetre. "Our ability to study them has been severely limited until now," explains Sylvia Schäffer from the Department of Biology at the University of Graz. Together with Stephan Koblmüller and her team, she has developed a procedure that allows researchers to obtain DNA samples in just a few steps. The bodies of the sensitive organisms are preserved for further analyses.

"Our method is a milestone for soil monitoring, which in turn will benefit many areas," says Schäffer. With their new procedure, the Graz biologists have already been able to obtain comprehensive DNA data for many previously unknown species, which is available in an international reference database. Based on this genetic information and the external shape of the animals, they can now be clearly identified and new species precisely described. The research results were published in the journal Geoderma.

Publication:
Sylvia Schäffer, Klaus Hasenhütl, Stephan Koblmüller: A window into dark taxa: morphology-compatible DNA extraction for tiny soil arthropods, Geoderma Volume 465.

microscope image of the pauropod Brachypauropus hamige
Now identified: the pauropod Brachypauropus hamige; Photo: Uni Graz/Klaus Hasenhütl
Microscope image of a horn mite
Also newly described: the horn mite Pterochthonius angelus; photo: University of Graz/Sylvia Schäffer
created by Dagmar Eklaude

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